Fig. 7: Retrotransposon mobilization in Mov10l1–/– testes at 9 d.p.p.
From: Formation of spermatogonia and fertile oocytes in golden hamsters requires piRNAs
a, Changes in RNAs from L1 and IAP families and subfamilies. RPM values of RNAs mapping to L1 or IAP elements (all), active subfamilies and FLI elements only are shown. Data are the mean values of two biological replicates. b, Immunofluorescence staining of IAP GAG (green) and γH2AX (red) in Mov10l1+/+ and Mov10l1–/– testes at 9 d.p.p. suggests IAP expression and DNA damage in germ cells in seminiferous tubules. Scale bars, 50 μm. c, 0 d.p.p. Mov10l1–/– testes show a normal presence of the germ cell marker DDX4 (VASA) and no mobilization of IAP expression. Four (b) and three (c) sections from one testis with a given genotype were individually stained. Representative images are shown. d, Changes in retrotransposon expression. The graphs rank the most upregulated LTR retrotransposon groups in Mov10l1–/– testes at 0 d.p.p. and 9 d.p.p. Values were calculated as RPM mean values from two RNA-seq libraries (Supplementary Table 10) from each time point and genotype. e, MYSERV and related RLTR31B2 LTR-derived transcripts are upregulated in Mov10l1–/– testes at 9 d.p.p. A UCSC browser snapshot shows a 3 Mb genomic region with upregulated retrotransposon loci (asterisks). Only perfectly mapping RNA-seq reads were used to construct the image.
