Extended Data Fig. 6: Genomic distribution of piRNAs at different stages.
From: The piRNA pathway is essential for generating functional oocytes in golden hamsters
(a) Genomic annotation of piRNA counts of different sizes identified in oocytes at the primary follicle stage through embryos at 52 h.p.e.a in wild-type and Piwil1m1/m1 golden hamsters. PF, primary follicle stage oocyte; SF, secondary follicle stage oocyte; GV, germinal vesicle stage oocyte; MII, metaphase II oocyte; 1 C, one-cell embryo; 2 C, two-cell embryo; 4 C, four-cell embryo. Piwil1m1/m1 indicates Piwil1-deficient oocytes or maternal Piwil1-deficient embryos. (b) Bar graphs show the distribution of piRNAs generated from different genomic regions. PIWIL1 29-nucleotide piRNAs, PIWIL1 23-nucleotide piRNA, and PIWIL3 19-nucleotide piRNAs were designated based on their location on the genome (piRNA cluster) and length, and were analyzed separately. Histograms left of the vertical dashed line show different families of repeat elements; histograms right of the vertical dashed line show the gene-related regions. The combination of Low complexity, Simple repeat, and Satellite is designated as LSS. Piwil1m1/m1 indicates Piwil1-deficient oocytes or maternal Piwil1-deficient embryos. Data shown a-b were average value of biological replicates at each time point: n = 3 (PF), 3 (SF), 3 (GV), 3 (MII), 3 (9 h.p.e.a.), 6 (33 h.p.e.a.), 4 (44 h.p.e.a.) or 3 (52 h.p.e.a.) for WT; n = 2 (PF), 2 (SF), 2 (GV), 2 (MII), 3 (9 h.p.e.a.), 2 (33 h.p.e.a.), 4 (44 h.p.e.a.) or 4 (52 h.p.e.a) for Piwil1 mutants.
