Extended Data Fig. 7: TE expression levels during oogenesis and embryo development.
From: The piRNA pathway is essential for generating functional oocytes in golden hamsters
(a) Volcano plots show the differentially expressed TEs between two adjacent stages during oogenesis and embryo development in wild-type golden hamsters. The highly significant up- or down-regulated TEs (≥ 2 folds; Welch two sample t-test, p-value < 0.05) are indicated in red or blue, respectively, with TE numbers shown at the top. PF, primary follicle stage oocyte; SF, secondary follicle stage oocyte; GV, germinal vesicle stage oocyte; MII, metaphase II oocyte. (b) Heat map of up-regulated TE expression levels in oocytes and embryos derived from WT and Piwil1-deficient females. The highly significant up-regulated TEs (≥ 2 folds) in Piwil1-deficient oocytes and maternal Piwil1-deficient embryos compared to wild-type oocytes and embryos at PF, SF, GV, MII, and 9 h.p.e.a. are plotted. Piwil1m1/m1 indicates Piwil1-deficient oocytes or maternal Piwil1-deficient embryos. Data shown were average value of biological replicates at each time point: n = 3 (PF), 3 (SF), 3 (GV), 3 (MII), 3 (9 h.p.e.a.), 6 (33 h.p.e.a.), 4 (44 h.p.e.a.) or 3 (52 h.p.e.a.) for WT; n = 2 (PF), 2 (SF), 2 (GV), 2 (MII), 3 (9 h.p.e.a.), 2 (33 h.p.e.a.), 4 (44 h.p.e.a.) or 4 (52 h.p.e.a.) for Piwil1 mutants.
