Extended Data Fig. 10: ZGA is impaired in maternal Piwil1-deficient embryos.
From: The piRNA pathway is essential for generating functional oocytes in golden hamsters
(a-b) Heat maps of gene expressions in oocytes and embryos derived from WT and Piwil1-deficient females. Only the genes which were up-regulated in wild-type embryos from 9 h.p.e.a. to 33 h.p.e.a. (a) or 33 h.p.e.a. to 44 h.p.e.a. (b) are shown. The up-regulation of gene expression in early embryogenesis of wild-type embryos was barely detectable in maternal Piwil1-deficient embryos. Data shown were average value of biological replicates at each time point: n = 3 (PF), 3 (SF), 3 (GV), 3 (MII), 3 (9 h.p.e.a.), 6 (33 h.p.e.a.), 4 (44 h.p.e.a.) or 3 (52 h.p.e.a.) for WT; n = 2 (PF), 2 (SF), 2 (GV), 2 (MII), 3 (9 h.p.e.a.), 2 (33 h.p.e.a.), 4 (44 h.p.e.a.) or 4 (52 h.p.e.a.) for Piwil1 mutants. PF, primary follicle stage oocyte; SF, secondary follicle stage oocyte; GV, germinal vesicle stage oocyte; MII, metaphase II oocyte. Piwil1m1/m1 indicates Piwil1-deficient oocytes or maternal Piwil1-deficient embryos. (c) Degradation of some maternal mRNAs is inhibited during the development of the Piwil1-deficient MII oocytes to maternal Piwil1-deficient 1-cell embryos at 9 h.p.e.a. and the maternal Piwil1-deficient 1-cell embryos at 9 h.p.e.a. to 2-cell embryos at 33 h.p.e.a. X- and Y-axes represent the log2 fold change of gene expression between adjacent developmental stages in WT (X-axis) or (maternal) Piwil1-deficient oocytes (embryos) (Y-axis), respectively.
