Extended Data Fig. 4: EETs amplify allergic asthma responses.
From: Eosinophil extracellular traps drive asthma progression through neuro-immune signals
a, Representative immunoblots of PAD4 and GAPDH in human eosinophils, neutrophils, monocytes, T cell, B cells and NK cells isolated from peripheral blood of healthy donors (n = 3). b, Representative immunofluorescence images of MPO, ECP, PAD4 and DAPI staining in human neutrophils and eosinophils isolated from peripheral blood of healthy donors. Scale bar, 50 μm. c, Quantification of Fig. 4b. d, Quantification of Fig. 4c. e, f, Wild-type and PAD4−/− mice were sensitized and challenged with PBS or OVA. PAD4−/− mice were adoptively transferred with 2×105 eosinophils or neutrophils isolated from wild-type or PAD4−/− mice. e, Quantification of Fig. 4e. f, Levels of IL-25, IL-33 and TSLP in BALF were evaluated by ELISA. c-f, Mean ± s.d.; n = 5 independent experiments (c, d); n = 6 mice per group, representative of two independent experiments (e, f); compared by one-way ANOVA with Tukey’s multiple comparisons test.
