Extended Data Fig. 5: EETs aggravate allergic inflammation by activating PNECs.
From: Eosinophil extracellular traps drive asthma progression through neuro-immune signals
a, Expression profiles of CCDC25 in different tissues from the FANTOM5 dataset. b, ECP+ H3cit+DAPI+ spots (0.1-μm-diameter) in lung sections of OVA-immunized mice were defined as EETs as shown in Fig. 5a. The density of EETs (number of spots per mm2) within 50 μm of PNECs, randomly selected blood vessels and airway walls (from epithelium to serosa, excluding the cartilage and 50-μm area surrounding PNECs) was quantified. c, CCDC25 expression level of CGRP+ and CGRP− cells in mouse lung sections was quantified by immunofluorescence as shown in Fig. 5b. d, Representative immunoblots of CCDC25 of lung homogenate from C57BL/6 mice challenged with PBS or OVA (n = 3). e-g, C57BL/6 mice immunized with OVA were treated without or with BIBN4096 or CGP35348. e, The inflammatory score quantified from H&E staining of lung sections. f, Levels of indicated type 2 cytokines in BALF evaluated by ELISA. g, Percentages of PAS-stained goblet cells in total epithelial cells in lung sections. h, The relative expression level of CGRP in H146 cells with indicated treatment was measured by qRT-PCR. i, Quantification of Fig. 5e. j, The relative level of intracellular Fluo-4 immunofluorescence intensity in live lung slices with indicated treatment was quantified at indicated time point. k, Quantification of Fig. 5h. b, c, e-k, Mean ± s.d.; n = 6 mice per group, representative of two independent experiments (b, c, e-g, k); n = 4 (h, i) or 3 (j) independent experiments; compared by two-tailed unpaired Student’s t-test (c) and one-way ANOVA with Tukey’s multiple comparisons test (b, e-k).
