Extended Data Fig. 2: BiFC/BiCAP approaches to assess the interaction between Rab21 and Swip1.
From: Cargo-specific recruitment in clathrin- and dynamin-independent endocytosis
(a) Cartoon of the BiFC/BiCAP approach used to image (BiFC) and biochemically detect (BiCAP) the interactions between Rab21 and Swip1 in live cells and from cell lysate. (b) MDA-MB-231 cells were transfected with the indicated constructs and lysed. Proteins expressed in the transfected cells were detected by immunoblotting. Immunoblot shows the size of the bands detected by a polyclonal anti-GFP antibody. Blots are representative of 2 independent experiments. (c) Representative confocal microscopy BiFC images of MDA-MB-231 cells expressing V1–Rab21 and V2–Swip1 MDA-MB-231 and immunostained for endosomal markers. Insets show BiFC colocalizing with the indicated endosomal markers. Scale bars, 10 µm (main figures) and 1 µm (insets). Representative pictures of 3 independent experiments. Unprocessed blots are provided in Source data.
