Extended Data Fig. 2: Daxx-deficient BM displays increased proliferation and altered stem/progenitor cell frequencies and numbers.
a, Genotyping of Daxx+/+;Mx1Cre+/- and DaxxF/F;Mx1Cre+/- mice untreated (-) or treated with pI:pC (+). Arrows indicate the wild-type, floxed and recombined alleles (n = 5 independent experiments). b, Daxx mRNA levels upon pI:pC treatment in bone marrow. Shown is relative expression of Daxx mRNA over Tbp mRNA levels (n = 3 mice per genotype). c, IF of Daxx in bone marrow, scale bar = 10 µm (n = 4 mice per genotype, two independent experiments). Inlay image zoomed on representative nuclei. d, Western blot of Daxx, alpha-tubulin and H3.3 in Lineage-negative and Lineage-positive bone marrow cells (n = 2 mice per genotype). e, Bone marrow cellularity counts before and after RBC lysis in male mice (n = 5 mice per gender, non-parametric Mann–Whitney test, box-and-whiskers plot: min to max (whiskers), 25th and 75th percentile and median). f, Bone marrow cellularity counts before and after RBC lysis in female mice (n = 5 mice per gender, non-parametric Mann–Whitney test, box-and-whiskers plot: min to max (whiskers), 25th and 75th percentile and median). g, Flow cytometry analysis of Ki-67+ cells in BM and HPCs (n = 5 mice per genotype, repeated in two independent experiments, non-parametric Mann–Whitney test). h,i, Flow cytometry analysis of HSC and MPP populations (n = 3 mice per genotype, repeated in two independent experiments, Student’s t-test). BM, bone marrow; HPC, haematopoietic progenitor cells; HSC, haematopoietic stem cell; MPP, multipotent progenitors. Data in box plots are mean and min to max. ns, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Exact p-values and numerical source data can be found in the accompanying source data. Unprocessed blots provided in Source data.
