Extended Data Fig. 4: Loss of Daxx leads to neutrophilia and B-cell lymphopenia in the periphery.

a, Flow cytometry analysis of peripheral blood collected 4 weeks post induction (n = 6 mice per genotype, repeated in three independent experiments). b, Cxcr2 levels in mature neutrophils of the blood (n = 3 mice per genotype, Student’s t-test). c, Quantification of WBC, RBC and platelets using Sysmex (n = 3 mice per genotype). d, IF images of spleen stained for Daxx and B220 (n = 2 mice per genotype, repeated in two independent experiments). Scale bar indicates 10 µm. e, Western Blot of Daxx protein in spleen (n = 3 mice per genotype, repeated in three independent experiments). f, H&E stain of spleen sections in Cre-negative animals, scale bar = 100 µm (n = 2 mice per genotype). g, Representative flow cytometry plots showing Gr-1⁺/Ly6C⁺ populations in bone marrow upon CD11b⁺, CD11c^-, Ly6G⁻, SSC^lo gating. h, Frequencies of Ki67⁺ cells in spleen (n = 5 mice per genotype, repeated in two independent experiments). i, Frequencies of erythroblast populations in spleen (n = 6 mice per genotype, repeated in three independent experiments). Data in box plots are mean and min to max. ns, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, Wilcoxon rank test if not otherwise noted. Exact p-values and numerical source data can be found in the accompanying source data. Source image file provided in Source data.

Source data