Extended Data Fig. 5: Loss of the H3.3 chaperone Hira has marginal effects on haematopoiesis.
a, Representative images of genotyping results using primers to detect WT, floxed and recombined bands of Hira in bone marrow cells (n = 3 independent experiments). b, Hira mRNA levels upon pI:pC treatment in bone marrow cells. Shown is relative expression of Hira mRNA over Tbp mRNA levels (n = 3 mice per genotype). c, Representative Western blots for Daxx and β-Actin in total bone marrow of Daxx +/+, Hira F/F and Daxx/Hira double KO Mx1Cre mice (n = 3 mice per genotype, repeated in two independent experiments). d, Bones of WT, Daxx KO and Hira KO mice. e, Frequencies of erythroblast populations in bone marrow (WT mice n = 9; Daxx KO n = 3; Hira KO n = 6). f, Flow cytometry analysis of mature cell markers in bone marrow (WT mice n = 9; Daxx KO n = 3; Hira KO n = 6). g, Flow cytometry analysis of B cells in bone marrow (WT mice n = 9; Daxx KO n = 3; Hira KO n = 6). h, Frequencies of neutrophils in bone marrow (WT mice n = 9; DKO n = 3; HKO n = 6). i, Frequencies of neutrophils in spleen (WT mice n = 9; Daxx KO n = 3; Hira KO n = 6). j, Spleens isolated from WT, Daxx KO and Hira KO mice. k, Flow cytometry analysis of mature cells in spleen (WT mice n = 9; Daxx KO n = 3; Hira KO n = 6). l, Flow cytometry plots gated on monocyte- and macrophage-like populations in spleen. m, H&E stain of spleen sections (n = 3 mice per genotype), scale bar = 100 µm. Higher magnification, scale bar = 20 µm. Data produced 3 weeks post pI:pC treatment. Data in box plots are mean and min to max. ns, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, Wilcoxon rank test. Exact p-values and numerical source data can be found in the accompanying source data. Source image file provided in Source data.
