Extended Data Fig. 1: Calu-3 cells bind Spike-RBD specifically and were engineered to express CRISPRi machinery enabling CRISPRi screening.
From: BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2
a, Spike-RBD binding in different cell types at 20 nM and 200 nM Spike-RBD was quantified by flow cytometry. b, Expression of CRISPRi machinery (dCas9-BFP-KRAB) in the CRISPRi Calu-3 line indicated by the expression of BFP by flow cytometry. C, Enrichment of sgRNAs targeting specific genes (coloured dots) or non-targeting control sgRNAs plotted against the negative log of the P-value with a FDR of 0.1 shown (dashed lines).
