Extended Data Fig. 7: Validation of dCas9–SSAP knock-in efficiencies in different cell lines.
From: dCas9-based gene editing for cleavage-free genomic knock-in of long sequences
(a–c) Results in HepG2, HeLa, and U2OS cell lines. The knock-in experiments used similar donor DNA with ~800-bp cassettes encoding 2A-mKate transgene for all cell lines tested. n = 2 biologically independent experiments. (d–f) Flow cytometry analysis of knock-in gene editing at HSP90AA1, ACTB, OCT4 endogenous loci in human embryonic stem cells (hESC, H9) using dCas9–SSAP compared with non-target controls and Cas9 (Cas9 HDR) references.
