Extended Data Fig. 4: Both the regulatory and trimerization domains are required for HSF1 phase separation. | Nature Cell Biology

Extended Data Fig. 4: Both the regulatory and trimerization domains are required for HSF1 phase separation.

From: Reversible phase separation of HSF1 is required for an acute transcriptional response during heat shock

Extended Data Fig. 4

(a) Domain structure of HSF1 truncations. Each amino acid number of the domain boundaries was labelled. (b) Confocal microscopy images of HeLa cells transfected with various HSF1 truncations as indicated under NHS and HS (42 °C, 0.5 h) conditions. Scale bars, 5 μm. (c) Representative images of HSF1 mutants under HS (42 °C, 0.5 h) condition before and after treatment with 10% 1,6-hexanediol for 1 min. Scale bars, 5 μm. (d) Co-localization analysis of HSF1△RD and stress granule marker G3BP1 under NHS and HS (42 °C, 0.5 h) conditions. Scale bars, 5 μm. Zoomed scale bars, 0.5 μm. (e,f) Co-localization analysis of TDP43 (e) and EWSR1 (f) with stress granule marker G3BP1 under NHS and HS (42 °C, 0.5 h) conditions. Scale bars, 5 μm. All Images in (b-f) are representative of 3 independent experiments.

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