Extended Data Fig. 7: LLPS-incompetent HSF1 mutant M3 were unable to form small condensates during HS. | Nature Cell Biology

Extended Data Fig. 7: LLPS-incompetent HSF1 mutant M3 were unable to form small condensates during HS.

From: Reversible phase separation of HSF1 is required for an acute transcriptional response during heat shock

Extended Data Fig. 7

(a) Super-resolution imaging of HSF1 M3 under NHS and HS (42 °C, 0.5 h) conditions. Scale bars, 5 μm. Zoomed scale bars, 0.2 μm. Images are representative of 3 independent experiments. (b-d) Cluster analysis of HSF1 M3 single molecules under NHS and HS conditions. Individual data points correspond to the average value for one cell. Cluster size (b), localization per cluster (c), and cluster number per cell (d) were shown. Data are presented as mean ± s.d. (d). n = 9 cells of WT NHS, 11 cells of WT HS, 17 cells of M3 NHS, and 18 cells of M3 HS pooled from 3 independent experiments. The paired two-tailed Student’s t-test was used to compare the data (d). Boxplots: 25th to 75th percentiles, median, 1.5×interquartile as whiskers (c).

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