Extended Data Fig. 4: VCAM1 is a “don’t-eat-me” signal.

(a) Contribution of 300 sorted DAPI^– LSK CD48^– CD150⁺ Vcam1⁺ (n = 8 biological replicates) or Vcam1^– (n = 7 biological replicates) HSCs to peripheral blood following competitive reconstitution. (b) Quantification of tri-lineage (myeloid, B lymphoid, and T lymphoid cells) engraftment 16 weeks post-transplantation. Vcam1⁺ (n = 8 biological replicates), Vcam1^– (n = 7 biological replicates). (c) Representative BM FACS plots showing donor HSC contribution to recipient CD45.2⁺ LSK CD48^– CD150⁺ Vcam1⁺ and Vcam1^– HSCs compartment, at 16 weeks from the mice analysed in (a) and (b). (d) Absolute number of donor (CD45.2) Vcam1^fl/fl and Vcam1^Csf1r-iCre cells that homed to the BM of lethally irradiated CD45.1 recipients, 3 hours after injection (n = 5 biological replicates). (e) Representative FACS plots of the in vivo phagocytosis assay in Fig. 1e. (f) Outline of experiment strategy. Lethally irradiated CD45.1 recipients were transplanted competitively with 2 million of Vcam1^Csf1r-iCre or Vcam1^fl/fl BMNCs. At day 6 the BM of recipient mice were analysed by FACS and the number of host phagocytic Gr1^high monocytes, Gr1^low monocytes and macrophages quantified. (n = 4 mice). Error bars, mean ± s.e.m. Box plots: media, whiskers: minimum and maximum. Unpaired two-tailed student’s t test. Significant P values are indicated in the figure.

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