Extended Data Fig. 3: Rod-shaped structure is a general feature of cellular necrosome.
From: Mosaic composition of RIP1–RIP3 signalling hub and its role in regulating cell death
a, Single-color STORM images of endogenous RIP3 complexes revealed by using an anti-RIP3 antibody. Magnified images outlined by white boxes emphasize the rod-shaped RIP3 complexes only present in WT but not RIP3-deficient HT-29 cells treated with TSZ for 8 h. b, STORM images of RIP1 or RIP3 in HT-29 cells treated with TSZ (T: TNF, 10 ng/ml; S: Smac mimetic (SM-164), 100 nM; Z: zVAD-fmk, 20 μM) for indicated time. Magnified images are shown as inset figures, highlighting the RIP1 or RIP3 clusters during cell necroptosis. c, Percentage of rod-shaped structures detected by RIP1 or RIP3 staining in total enlarged clusters (RIP1, n = 1, 277, 334 for TSZ 0, 4, 8 h, respectively; RIP3, n = 8, 468, 409 for TSZ 0, 4, 8 h, respectively) by TSZ stimulation. The definitions of round- and rod-shaped structures are described in Extended Data Fig. 2d,e. d-g, Width (d) and length (f) distributions of RIP1 stained rods (n = 43, 61 for TSZ 4, 8 h, respectively) or width (e) and length (g) distributions of RIP3 stained rods (n = 76, 105 for TSZ 4, 8 h, respectively) in necroptotic HT-29 cells treated as in b. In c-g, ‘n’ refers to the number of structures analyzed across three independent experiments. Scale bars, 5 μm (original images in a and b) and 100 nm (magnified images in a and b). Statistical source data are provided in Source Data Extended Data Fig. 3.
