Extended Data Fig. 9: The effects of expression of mRIP3 WT or mutants in HEK293T cells.
From: Mosaic composition of RIP1–RIP3 signalling hub and its role in regulating cell death
a-c, Flag-mRIP3 WT or mutants (K51A, D161N, or RHIMMut) were expressed in HEK293T cells with or without 5 μM GSK’872. Confocal (a) and STORM (c) images of RIP1-RIP3 complexes are shown. The fraction of cells with RIP3 puncta in a were calculated (b). n = 97–156 cells pooled by two independent experiments. Data in c are representative of three independent experiments. Scale bars, 10 μm (a) and 100 nm (c). Statistical source data are provided in Source Data Extended Data Fig. 9.
