Extended Data Fig. 5: Dual antibiotic selection for co-transfected mouse P19 cells. | Nature Cell Biology

Extended Data Fig. 5: Dual antibiotic selection for co-transfected mouse P19 cells.

From: Multiplexed genome regulation in vivo with hyper-efficient Cas12a

Extended Data Fig. 5

a, Mouse P19 cells were co-transfected with constructs expressing puromycin resistance (PuroR) and hygromycin resistance (HygroR), then selected with puromycin and hygromycin at 24 hr after transfection. Cells were collected for analysis 72 hr after transfection. b, Histograms showing percentage of BFP + (crRNA) and mCherry + (dCas12a) cells for non-transfected, non-selected, and Puro/Hygro-selected cells. c, Flow cytometry plots. Data in panels b-c are representative plots of n = 3 independent experiments.

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