Extended Data Fig. 5: RNA is clustered at the edges of pre-formed condensates.
From: Membrane surfaces regulate assembly of ribonucleoprotein condensates
(a) Time series of two example condensates formed in the presence of 50 nM Whi3 and 100 pM CLN3. Images show condensates formed in proximity to an RNA puncta (black dashed box) and with no associated RNA (gray dashed box). (b) Time-lapse of condensate assembly on SLB with 50 nM Whi3 and 100 pM CLN3. Frames span 2.5–8.5 min after addition of Whi3, 1 min between frames. White arrows indicate Whi3 condensates visible after 3.5 min, and yellow arrowheads indicate condensate edge-associated CLN3 puncta 1 min later. White asterisks in final frames indicate bright CLN3 clusters that continued to assemble at condensate edges. (c) Intensity histograms of CLN3 puncta adsorbed to condensate edges or in the dilute phase on the surrounding membrane, 20–30 min after addition of 50 nM Whi3. n = 467 and 24,954 edge-adsorbed and dilute phase puncta, respectively, from 4 biologically independent samples. p-value from two-sided Kolmogorov-Smirnov test. (d) Membrane-associated condensates do not attain measurable height. Maximum intensity projections from a spinning disc confocal z-stack of condensates formed in the presence of 500 nM Whi3, with z-spacing of 0.2 µm. SLB membrane composition: 96 mol% DOPC, 4 mol% DGS NTA-Ni.
