Extended Data Fig. 2: MS-identified O‑GlcNAcylation of RYR1 and SERCA3 in skeletal muscle.
From: Cancer-cell-secreted miR-122 suppresses O-GlcNAcylation to promote skeletal muscle proteolysis
(a) Western blots showing O‑GlcNAcylation in lung, liver, adipose tissue, kidney, and brain of tumour-free and tumour-bearing mice described in Fig. 1e. (b) Western blots of GA from mice that have received repeated injections of indicated EVs as in Fig. 1e. (c) The MS/MS spectra of O-GlcNAcylated peptides of mouse RYR1 (residues 4324–4353) and SERCA3 (residues 534–550) with the triply charged precursor ion m/z 638.9881 (M + 3H)3+. The c- and z-type product ions were assigned. The O-GlcNAc oxonium ion (m/z, 204.09) and a series of its fragments (m/z, 186.08, 168.06, 138.05, and 126.05) were also assigned. (d) OGTSite analysis predicting the herein identified O-GlcNAcylation sites in RYR1 and SERCA3 of human and mouse.
