Extended Data Fig. 1: NANOG NTD and CTD characterization. | Nature Cell Biology

Extended Data Fig. 1: NANOG NTD and CTD characterization.

From: NANOG prion-like assembly mediates DNA bridging to facilitate chromatin reorganization and activation of pluripotency

Extended Data Fig. 1: NANOG NTD and CTD characterization.

a, NANOG WR sequence conservation. Protein sequence alignment of NANOG WR domains using BioEdit ClustalW73. The sequence origins are: human (NP_079141.2), house mouse (NP_082292.1), chimpanzee (NP_001065295.1), dog (XP_025327655.2), chicken (NP_001139614.1), and zebrafish (NP_001091862.1). The relatively conserved hydrophobic residues tryptophan, tyrosine, and phenylalanine are highlighted in light blue. b, CD spectra of NANOG NTD (100 μM) show random coil signature. Similar results were observed for 2 independent experiments. c, NANOG predicted disorder. Domain organization of NANOG (top) and the disordered region prediction (bottom) by PONDR VS-L21 (www.pondr.com).

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