Extended Data Fig. 4: DNA methylation asymmetry at allele-specific CHMs influenced by H3K9me3 depletion. | Nature Cell Biology

Extended Data Fig. 4: DNA methylation asymmetry at allele-specific CHMs influenced by H3K9me3 depletion.

From: Allele-specific H3K9me3 and DNA methylation co-marked CpG-rich regions serve as potential imprinting control regions in pre-implantation embryo

Extended Data Fig. 4

a, Representative immunofluorescence staining for H3K9me3 (green), DAPI (grey) and merged images in control and H3K9me3-depleted embryos at the zygote stage. Data is reproducible in three independent experiments. Scale bar, 20 μm. b, The UCSC Genome Browser view of a representative mCHM showing the decreased DNA methylation level in H3K9me3-depleted embryos at the morula stage. mCHM is indicated with orange rectangles. DNA methylation change after H3K9me3-depleted represents smoothed DNA methylation level change (H3K9me3-depleted - WT) in 200-bp windows.

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