Extended Data Fig. 2: 3’UTR splicing is upregulated in cancer and correlated with poor prognosis.

a, Workflow of the analysis for each c3USP in each cancer type. b, Bar plot showing the number of genes with up and downregulated c3USPs. c, Bar plot illustrating the proportion of oncogenes and tumor suppressors in two groups of genes with either upregulated or downregulated c3USPs. P-values: hypergeometric test. d, Heatmap illustrating p-values derived from overlapping c3USPs between each two cancer types (hypergeometric test). P-values >0.001 are labeled. e, Bar plot showing the number of favorable and unfavorable prognostic c3USPs across different cancer types derived from the Kaplan-Meier method with a p < 0.05 cutoff. f, Bar plot illustrating the proportion of favorable and unfavorable markers in upregulated and downregulated c3USPs. g-i, Sashimi plots illustrating 3’UTR splicing of the top dysregulated splicing event of CTNNB1 in the normal and tumor samples from AML (g), TCGA-BRCA (h) and -LIHC (i). j, Number of 3’UTR splicing events in all (left), and 50 matched tumor-normal samples (right) from the TCGA-LIHC dataset. n: number of RNA-seq samples analyzed; P-values: Mann-Whitney U test. k, Kaplan-Meier survival analysis of TCGA-LIHC samples based on the segmentation numbers of total splicing events (top and bottom half of samples ranked by numbers). n: number of patients analyzed.