Extended Data Fig. 4: Targeted inhibition of 3’UTR splicing impedes HCC cell proliferation.
From: Pan-cancer pervasive upregulation of 3′ UTR splicing drives tumourigenesis
a-e, Effect of ASO-mediated blocking of the 3’UTR splice site on the protein expression of cell cycle markers (a), candidate transcript expression by qPCR (n = 3 independent experiments) (b), and PCR (◄ 3’SP2, see Supplementary Note) (c), candidate protein expression (d) and anchorage-independent growth (n = 3 independent experiments) (e) in Hep3B and HepG2. CDS: coding sequence; 3’FL: full length 3’UTR; 3’SP: spliced 3’UTR; ASO-NC: non-targeting control ASO; ASO-SS: splice site ASO. b,e, Mean ± SEM; unpaired Student’s t-test *p < 0.05, **p < 0.01, ***p < 0.001. a,c,d, Data shown represent three independent experiments.
