Fig. 6: 3′ UTR splicing may enhance CTNNB1 expression to promote tumourigenesis.
From: Pan-cancer pervasive upregulation of 3′ UTR splicing drives tumourigenesis
a, Volcano plot showing changes in CTNNB1 3′ UTR splicing upon RBP knockdown. Putative binding was predicted using eCLIP-seq data. b,c, Effect of splicing RBP knockdowns on CTNNB1 transcript (n = 3 independent experiments) (b) and protein (c) expression in Hep3B. d, Enrichment of CTNNB1 transcripts by RBP RIP in Hep3B and SNU398 (n = 4 independent experiments). e, Enrichment of RBPs upon CTNNB1 3′ FL and 3′ SP pulldown using biotinylated probes. f, Effect of overexpressing CTNNB1 CDS, CDS + 3′ FL and CDS + 3′ SP on exogenous CTNNB1 protein expression. g,h, Luciferase activity of plasmid-transfected (n = 4 independent experiments) (g) and RNA-transfected (n = 5 independent experiments) (h) CTNNB1 reporter constructs. i, Polysome profiles for CTNNB1 3′ FL and 3′ SP in Hep3B and SNU398 (n = 2 independent experiments). HSP90 and TBP are housekeeping controls. S, sense; AS, antisense; EV, empty vector. In b, d, g and h: mean ± s.e.m.; unpaired Student’s t-test *P < 0.05, **P < 0.01 and ***P < 0.001. In c, e and f: data represent three independent experiments.
