Fig. 7: 3′ UTR splicing-mediated cytoplasmic localization may enhance CTNNB1 expression.
From: Pan-cancer pervasive upregulation of 3′ UTR splicing drives tumourigenesis
a, Enrichment of the CTNNB1 3′ FL transcript by U1 snRNP RIP (n = 3 independent experiments). b, Subcellular distribution of CTNNB1 3′ FL and 3′ SP transcripts following nuclear–cytoplasmic fractionation of Hep3B and SNU398 cells. MALAT1 was used as a nuclear control. The 3′ FL:3′ SP transcript ratios in each cellular compartment are presented in the table below (n = 4 independent experiments). c, RNA–FISH showing transcript localization of CTNNB1 3′ FL and 3′ SP in Hep3B and SNU398. Data represent three independent experiments. In a and b: mean ± s.e.m.; unpaired Student’s t-test *P < 0.05, **P < 0.01 and ***P < 0.001.
