Fig. 8: Upregulated 3′ UTR splicing may promote carcinogenesis.
From: Pan-cancer pervasive upregulation of 3′ UTR splicing drives tumourigenesis
a, Effect of overexpressing CTNNB1 CDS + 3′ FL-WT, CDS + 3′ FL-5′ SSmut and CDS + 3′ SP on exogenous CTNNB1 protein expression. Data represent three independent experiments. b,c, Luciferase activity of plasmid-transfected (n = 4 independent experiments) (b) and RNA-transfected (n = 5 independent experiments) (c) CTNNB1 3′ FL-WT and 3′ FL-5′ SSmut reporter constructs. d, Effect of ASO-mediated blocking of the CTNNB1 3′ UTR splice site on the polysome profiles for CTNNB1 (n = 2 independent experiments). HSP90 and TBP are housekeeping controls. e, Schematic depicting how increased 3′ UTR splicing may promote carcinogenesis. In b and c: mean ± s.e.m.; unpaired Student’s t-test *P < 0.05, **P < 0.01 and ***P < 0.001.
