Extended Data Fig. 4: m6A profiles of TEs.
a, PCA showing m6A IP and input samples at all repetitive RNAs during MZT. Squares are input samples, and circles are m6A samples. b, Average RNA level of ERV, L1 and MaLR during MZT. c, Bar plots showing average RNA level (RPKM) for maternal expressed TEs in GV, MII (left) and zygotic expressed TEs in L2C and 4 C (right). d, Bar plots showing genomic copy number for maternal expressed TEs (left) and zygotic expressed TEs (right). e and f, Unmodified MTA(e) and MERVL(f) copies are not expressed or of low expression level in GV oocytes and 2-cell embryos. Full-length TE copies were classified into five groups according to expression levels. “1” represents copies with the lowest RNA level, while “5” represents copies with the highest level. Each group of copies was further classified according to m6A status. g and h, Sequence logo and p-values of the consensus motif of m6A peak centers at the MTA(g) and MERVL(h) locus. i and j, The UCSC browser track showing IP and input reads of MTA(i) and MERVL(j) during MZT. k, Average profile of m6A IP and input signal calculated by unique mapped reads for full-length MTA copies during MZT. l, The UCSC browser track showing example of MTA using only the unique mapped reads. m, Average profile of m6A IP and input signal calculated by unique mapped reads for full-length MERVL copies during MZT. n, The UCSC browser track showing example of MERVL using only the unique mapped reads during MZT. Data in a-n represent results from three independent IP experiments of GV to 2-cell and ESC, and two independent IP experiments of 4-cell.
