Extended Data Fig. 6: Potential roles of YTHDF2 and IGF2BP family proteins in oocytes.
a, Schematic diagram of RNA-seq for early embryos using Ythdf2 Zp3-cKO mice. b, Immunostaining of YTHDF2 in Ythdf2 Ctrl and Zp3-cKO GV oocytes. Scale bar = 20 μm. c, PCA of RNA-seq for MII oocytes and 2-cell embryos of Ythdf2 cKO and control mice. d, Heatmap of normalized expression levels of maternal decay genes for MII oocytes and 2-cell embryos in control and Ythdf2 cKO mice. The classification of M-decay and Z-decay transcripts for Ythdf2 cKO samples are according to single-cell RNA-seq data. Gene numbers were labeled in parentheses. e, Gene expression level (FPKM) from RNA-seq data showing Igf2bp1/2/3 expression level during oocyte and early embryo development. f, RIP-qPCR showing fold enrichment of IGF2BP2 and IGF2BP3 at MTA and SINEb1 RNAs over IgG. Data shown represent 2 biological replicates for IGF2BP3-RIP and 1 biological replicate for IGF2BP2-RIP; value for each biological replicate represents the mean of two technical replicates. g, MTA expression level relative to Actin in GV oocytes of Igf2bp2 WT and KO mice. Data shown represent 2 biological replicates; value for each biological replicate represents the mean of two technical replicates. Data in c and d, n = 5 biological replicates of MII oocytes and Ctrl 2-cell embryos. n = 4 biological replicates of Ythdf2 cKO 2-cell embryos.
