Extended Data Fig. 3: m6A dynamics during MZT.
a, The number of transcripts harboring m6A (m6A + ) in each stage. b, Alluvial diagram showing the global dynamics of m6A + transcripts during MZT(n = 6940). Each line represents a transcript that is classified as a m6A + class in at least one stage. c, Bar chart showing m6A peak number in each sample. d, Alluvial diagram showing the global dynamics of m6A peaks during MZT (n = 43980). Each line represents a m6A peak in at least one stage. e, Heatmap showing normalized gene expression (left panel) and m6A modification status (right panel) of transcripts which gained m6A modification at L1C and L2C compared to GV and MII. f, The UCSC browser track showing IP and input reads of MD transcripts examples. g, The UCSC browser track showing IP and input reads of ZGA transcripts examples. h, Box plot showing [RRACH] motif density of ± 200 bp of stop codon in MD transcripts and ZGA transcripts with and without m6As. Box plots are presented with horizontal line, median; box, interquartile range (IQR); whiskers, most extreme value within ±1.5 × IQR. Two-tailed unpaired Wilcoxon test was used to calculate the p-values. i, Pie plot showing M-decay and Z-decay transcripts ratios of MD transcripts. Data in a-i represent results from three independent IP experiments of GV to 2-cell and ESC, and two independent IP experiments of 4-cell. Peaks of each stage are the intersect peaks of replicates or triplicates.
