Fig. 2: Ede1 regulates proteasome assembly upon TORC1 inhibition.
From: Actin remodelling controls proteasome homeostasis upon stress
a, Cells spotted in a fivefold dilution and grown for 3 days on plates with or without 20 ng ml−1 rapamycin. b, Western blot analysis of RPACs in WT and deletion strains that were untreated or treated with 200 nM rapamycin (Rapa) for 4 h. Ponceau S staining was used as a loading control. Asterisk indicates non-specific band. Data are representative of three independent biological replicates. c, Gradient native polyacrylamide gel electrophoresis (PAGE) (3.8–5%) of yeast extracts from untreated cells or cells treated with 200 nM rapamycin (Rapa) for 3 h, monitored by the fluorogenic substrate Suc-LLVY–AMC (left) and by immunoblots (right). CP, single-capped (RPCP), double-capped (RP2CP) and Blm10-capped (Blm10-CP) proteasome complexes are indicated. Rpt5 and 20 S antibodies recognize the RP and the CP, respectively. Data are representative of three independent biological replicates.
