Extended Data Fig. 8: Inhibition of YAP abrogates Thy1-/–dependent phenotypes.
From: THY1-mediated mechanisms converge to drive YAP activation in skin homeostasis and repair
a, WT and Thy1-/- dorsal skins treated with 2.5% DMSO, and Thy1-/- treated with verteporfin (vert) at 36 h post BrdU (n = 9 mice per treatment). b, Number of BrdU+ cells from WT (DMSO-treated), Thy1-/- (DMSO-treated) and Thy1-/- (Vert-treated) mice (n = 9 mice per treatment). c, Schematic representation of YAP inhibition and dorsal wound infliction. d, Photographs of WT dorsal wounds treated with DMSO or Vert at day 7 post wound infliction (PWI). e, Re-epithelialization dynamics of WT skins. Percentage of wound coverage was calculated versus original wound size (n = 4 mice). f, Immunostaining against MCM2 and ITGβ4 (CD104) in dorsal wounds from WT (DMSO), Thy1-/- (DMSO) and Thy1-/- (Vert) mice. g, Percentage of MCM2+ suprabasal cells in treated mice (n = 4 mice). h, WT (DMSO), Thy1-/- (DMSO) and Thy1-/- (Vert) treated dorsal skins immunostained for MCM2 at day 7 PWI. i. Number of de novo hair follicles in the dorsal wound bed from WT (DMSO), Thy1-/- (DMSO) and Thy1-/- (Vert) mice at 7 days PWI (n = 4 mice). j. Schematic representation of ITGβ1 inhibition and dorsal wound infliction. k, WT dorsal skin treated with IgG or ITGβ1 antibody (ITGβ1 Ab) immunostained for active YAP and Ki-67 at day 7 PWI. l, Relative percentage active YAP+ cells in suprabasal layer from WT and Thy1-/- mice treated with IgG or ITGβ1 Ab at day 0 PWI (n = 4 mice). m. Dorsal skin sections from WT and Thy1-/- mice treated with IgG or ITGβ1 Ab immunostained for active YAP and Ki-67 at day 7 PWI. n-o. Quantifications of active YAP+ (n) and Ki-67+ (o) basal cells from WT and Thy1-/- mice treated with IgG or ITGβ1 Ab at day 7 PWI (n = 3 mice). p-q, Quantifications of active YAP+ (p) and Ki-67+ (q) suprabasal cells from WT and Thy1-/- mice treated with IgG or ITGβ1 antibodies at day 7 PWI (n = 3 mice). All images and quantifications are representative of at least n = 6 mice per genotype and treatment, unless otherwise stated. Error bars indicate mean ± s.e.m. Scale bars, 50 μm (a,f,h,k,m).
