Extended Data Fig. 6: Zic2 and Zic3 cooperate with Mesp1 and potentially with other mesoderm-inducing TFs.
a, Western blot illustrating the expression of Zic2 and Zic3 with and without Mesp1 induction, at day 3.5 (24 h) of PSC differentiation, as well as the lack of Zic2 and Zic3 protein expression in Zic2/3 double KO cell lines. (Data shown represent 2 independent experiments) b, Illustration of the two most enriched motifs in all Zic2 and Zic3 ChIP-seq detected peaks. p-values are calculated through a binomial test. c, Venn diagram illustrating the number of overlapping peaks between Mesp1, Zic2 and Zic3 ChIP-seq datasets. P-value was calculated using a hypergeometric test, using bedtools fisher. d, Illustration of the temporality of Zic3 binding within primed and de novo Mesp1-bound peaks, with associated ATAC-seq signal. 1, peaks already bound by Zic3 at day 2.5 (0 h); 2, peaks bound by Zic3 at 24 h no dox; 3, peaks bound by Zic3 at 24 h dox.
