Extended Data Fig. 2: Increased innate immune signaling in Abro1-deficient cells depends on DNA2 but not MRE11.
From: Stalled replication fork protection limits cGAS–STING and P-body-dependent innate immune signalling
(a) Inhibition of Mre11 does not decrease pSTAT levels in Abro1 KO cells. Cells were treated with 4 mM HU in the absence or presence of Mirin. (b) Inhibition of Mre11 does not decrease cytosolic ssDNA in Abro1 KO cells. Immunofluorescence was performed with ssDNA antibody. Scale bars, 10 μm. Quantification of cytosolic ssDNA (mean fluorescence intensity, MFI) is shown with mean ± SD. One way Anova was used for statistics. ‘n’ refers the number of cells analysed across three independent experiments. (c) Knockdown of RAD51 decreases pSTAT1 levels in Abro1 KO cells. Cells were treated with 4 mM HU. Data shown represent three independent experiments in a and c.
