Extended Data Fig. 5: Effect of GsMTx4 on hypotonically-induced [Ca2+] changes.
From: The spectrin cytoskeleton integrates endothelial mechanoresponses
![Extended Data Fig. 5: Effect of GsMTx4 on hypotonically-induced [Ca2+] changes.](http://media.springernature.com/full/springer-static/esm/art%3A10.1038%2Fs41556-022-00953-5/MediaObjects/41556_2022_953_Fig12_ESM.jpg?as=webp)
Live imaging of confluent endothelial cells expressing the cytosolic [Ca2+] indicator GCaMP6 before and after the introduction of hypotonic stress (grey background). Images were acquired every 12 s. Cells were treated with vehicle (PBS) or the small peptide GsMTx4 for 30 min prior to imaging. Quantification of mean GCaMP6 fluorescence over time. Data are means ± SE (shaded area), n = 3 independent experiments, quantifying 12,8,9 cells per condition.
