Extended Data Fig. 4: Window of active labelling of DOX in vivo.
From: Murine foetal liver supports limited detectable expansion of life-long haematopoietic progenitors
A. Experimental schematic. ROSA26 rtTa/+ Col1a1tetO-H2B-GFP/+ CD45.2+ donor BM68 was pooled from five donors and transplanted into CD45.1+/CD45.2+ recipient mice previously treated with DOX on day three (-3), two (-2) or one (-1) before transplantation or on the same of transplant (day 0) (n = 3 recipients per group). B. %GFP+ BM of recipients 4, 7, or 11 days post-transplant (Trx). C. To corroborate the ability of non-labelled transplanted CD45.2+ cells to respond to DOX, CD45.2+ c-Kit+ sorted cells from each mouse cohort were cultured in vitro in the presence or absence of DOX showing that cells were responsive to DOX in vitro.
