Extended Data Fig. 5: Fetal liver factor ANGPTL3 is not able to promote self-renewing expansion of E14.5 FL-HSCs.

A. Gating strategy on E12.5-CD45⁺c-Kit⁺ cells and E14.5-c-Kit⁺ and E14.5-HSC cells. Related to Fig. 3B-C & 4. B-D. LSK CD150⁺CD48⁻C57Bl/6 HSCs were isolated from E14.5 FL or adult BM and cultured in PVA cultures tailored for self-renewing expansion either with or without addition of ANGPTL3, for 2 weeks. Immunophenotypic HSC expansion was then quantified. Due to known immunophenotypic shifts during PVA culture, HSC were defined as LSK CD150⁺EPCR⁺ after culture. B. Experimental schematic. C. Gating strategy of LSK CD150⁺EPCR⁺ HSC after culture. D. Proportion of wells expanding, defined as containing at least 100 immunophenotypic LT-HSCs. E. For those wells showing cell expansion, HSC expansion as a ratio of output/input is shown. For each condition, 5 biological replicates and 2 independent experiments with 10 wells/replicate. Each circle/square represents an individual biological replicate. Means and standard deviations are depicted. For (D), the Holm-Sidak method (2-tailed) was used to calculate statistical significance and correct for multiple comparisons. Exact p-values are shown in Figure.

Source data