Extended Data Fig. 4: Elp3 depletion induces a short bristle phenotype.
(a) Scanning Electron Microscopy of the notum of representative control, Elp3 RNAi and Elp3 RNAi + Elp3* flies. Dashed lines indicate panier expression region. (b) Microchaete length in the panier region (Mean ± SEM). Statistics: Kruskal–Wallis test (p-value as indicated; n: microchaete number). Note the smaller bristles in the panier region in the Elp3 RNAi genotype. This is rescued by expression of a RNAi-resistant wild-type version of Elp3 (Elp3*). (c) Dividing SOP showing mRFP-Pon after immunolabelling with antibodies against endogenous Elp2 and α-tubulin. Images corresponds to single confocal plane (top panels) or maximum intensity z-projection of two planes (point scanning confocal microscopy). For mRFP-Pon: safir denoising filter (see methods). Top panel displays cell in metaphase and bottom panel a cell in early anaphase. Note that the cell on the bottom panel is at a too early stage of anaphase to display central spindle asymmetry, which gradually builds up during anaphase B (see Fig. 1a). (d,e) Fly nota were stained for tubulin and endogenous Elp2 (d) or Elp4 (e) to image epithelial cells (that is, non-SOP cells). Images corresponds to a maximum intensity z-projection (point scanning confocal microscopy). Both Elp2 and Elp4 antibodies label the mitotic spindle (orange arrow) and central spindle (blue arrow) in epithelial cells. Scale bar: 100 µm (a) and 5 µm (c-e). Source numerical data are available in source data.
