Fig. 3: RDE-1-dependent RNA turnover is affected by ER stress.
From: ER-associated RNA silencing promotes ER quality control
a, RNA sequencing results of cpl-1* expressing worms compared with WT and cpl-1WT expressing worms, respectively. The cut-off for filtering of upregulated constructs was performed with P ≤ 0.05 (brown spheres). b, GO term enrichment for biological processes of the transcripts identified in a (brown). Only transcripts that were upregulated in both datasets shown in a were selected for analysis, resulting in 996 upregulated transcripts. c,d, α-Amanitin chase assays for cpl-1WT and cpl-1* mRNA from mutant animals for ERAD and/or ERAS expressing cpl-1WT or cpl-1* mRNA measured by qRT–PCR. Data relative to 18S rRNA, cdc-42 and pmp-3 mRNA. e, hsp-4 mRNA level measured by qRT-PCR at the 0 h timepoint corresponding to the samples shown in d. Data relative to cdc-42 and pmp-3 mRNA. In c and d, data were analysed by two-way ANOVA with Dunnett’s multiple comparison test. In e, data were analysed by one-way ANOVA with Sidak’s multiple comparison test. In c–e, values are depicted as mean ± s.e.m. generated from n = 3 independent experiments, *P < 0.05; **P < 0.01; ***P < 0.001; NS, P > 0.05. In b, raw P values were corrected for FDR. *FDR <0.05; **FDR <0.01; ***FDR <0.001; NS, FDR >0.05. Source numerical data are available in source data.
