Extended Data Fig. 1: CPL-1* model substrate to monitor ER quality control in C. elegans.
From: ER-associated RNA silencing promotes ER quality control
(a) Schematic overview of mutations inserted into the CPL-1WT encoding transgene for expression of the CPL-1* ERAD substrate. (b) Schematic overview of the CPL-1* model substrate for studying ERQC. (c) Fluorescence images of worms with mutations in ERAD components expressing cpl-1*; C47E12.3 (EDEM-1 homologue), empty vector (empty). (d) Western blot of worm lysates corresponding to samples shown in (c), detecting CPL-1* (YFP) and tubulin (TUB). (e) Fluorescence images of worms expressing cpl-1* with defects in autophagy. (f) Western blot of worm lysates corresponding to the samples in (e), detecting CPL-1* (YFP) and tubulin (TUB). (g) Schematic overview of different sel-11 alleles used in this study; nDf59: deletion allele, hh17: Introduction of a stop codon at amino acid position 322 of the SEL-11 protein. [(c), (e)] Pharyngeal expression of Pmyo-2::mCherry serves as transgenic marker. Scale bar: 200 µm. WT: wild-type. Unprocessed blots are available in the source data.
