Extended Data Fig. 2: Monocytes depletion by alendronate impairs angiogenesis.
From: Monocytes deposit migrasomes to promote embryonic angiogenesis
(a) After treatment with control liposomes or alendronate liposomes, CAM9d were stained by CD115-Alexa488 to detect monocytes inside and outside the blood vessels. Inside vessels: CD115-Alexa488 was microinjected into the CAM vasculature. Outside vessels: CD115-Alexa488 was add onto the CAM surface. Images were taken 30 minutes after CD115-Alexa488 staining. Scale bar, 50 μm. (b-c) The numbers of monocytes inside or outside vessels were quantified according to the CD115 signal. Data are presented as mean ± SEM; n = 32 samples from three independent experiments; P values were calculated using a two-tailed, unpaired t-test, P < 0.0001. (d) CAMs treated with control liposomes and alendronate liposomes were visualized with a stereomicroscope (left panel) or a confocal microscope after dextran-FITC microinjection (right panel). Scale bar, left panel, 500 μm; right panel, 50 μm. (e) The number of sprouting capillaries from (d) was quantified according to the intensity of the dextran signal. Data are presented as mean ± SEM; n = 32 samples from three independent experiments; P values were calculated using a two-tailed, unpaired t-test, P < 0.0001. Source numerical data are available in source data.
