Extended Data Fig. 5: Adenoviral CerS6 overexpression does not affect Pcsk1 levels.
From: Sphingolipid subtypes differentially control proinsulin processing and systemic glucose homeostasis
a, Immunoblot detection of human CerS6 (hCerS6) and Tmed2 protein levels in INS1E cells after overexpression of hCerS6 via adenovirus for 48 h (representative immunoblot). b, Ins1E cell counts after 48 hours of infection with a control adenovirus or hCerS6-expressing adenovirus (n = 3 independent experiments). A reduction of cell counts is in line with the ability of CerS6 overexpression to induce apoptosis in several cell types. c, Immunoblot detection of Pro-Pcsk1 and Pcsk1 protein levels (Cell Signaling #18030) in Ins1E cells infected with a control adenovirus or hCerS6-expressing adenovirus for 48 h (representative immunoblot). d-g, Quantification of Tmed2 (d), Pcsk1 (e), Pro-Pcsk1 signals (f) and Pcsk1/ Pro-Pcsk1 ratio (g) from 4 independent experiments. Statistical analysis was performed using two-way ANOVA with Sidak’s multiple comparisons test (b, d-g). Data points in (b, d-g) represent independent experiments. Bar graphs in (b, d-g) represent means + s.e.m. Stain-Free signal was used for normalization of all immunoblots. Source numerical data and unprocessed blots are available in source data.
