Extended Data Fig. 10: Interaction and localization of overexpressed Tmed2 and Pcsk1 in Ins1E cells.
From: Sphingolipid subtypes differentially control proinsulin processing and systemic glucose homeostasis
a, Co-immunoprecipitation (Co-IP) of co-overexpressed Tmed2-V5 and Pro-Pcsk1/Pcsk1-DDK in Ins1E cells. Representative immunoblot (left) and quantification of three replicate experiments (right). As Ctrl-plasmid, the promotorless pNL1.3 from Promega (N1021) was used. b, Representative confocal images for co-localization of overexpressed Tmed2-V5 and Pro-/Pcsk1-DDK in Ins1E cells. Green, SytoxGreen as nucleus marker; red, Pro-/Pcsk1-DDK; blue, Tmed2-V5. Scale bar, 5 µm. c, d, Quantification of overlap of Pro-/Pcsk1-DDK with Tmed2-V5 (c) and Tmed2-V5 with Pro-/Pcsk1-DDK (d) in control and CerS2ΔIns1E cells. n = 2 independent experiments; only one experiment shown. e-j, Overlap of ER-marker PDI and Golgi-Marker TGN46 with Tmed2-V5, Pro-/Pcsk1-DDK (allowing detection of both Pro-Pcsk1 as well as mature Pcsk1) and Pro-Pcsk1 (only allowing detection of the immature Pro-Pcsk1 protein) after overexpression in control and CerS2ΔIns1E cells. n = 3 independent experiments. Statistical analysis was performed using a paired two-sided Student’s t-test (a) and unpaired two-sided Students t-tests (c-j). Data points represent replicate experiments (a) and individually quantified cells (c-d) or well sites (e-j). Bar graphs represent means + s.e.m. Source numerical data and unprocessed blots are available in source data.
