Extended Data Fig. 2: The islet sphingolipidome of Akita and ob/ob.B6 mice.
From: Sphingolipid subtypes differentially control proinsulin processing and systemic glucose homeostasis
a, b, Body weight (a) and blood glucose levels (b) of 13 male control and 14 ob/ob.B6 mice at week 12. c-h, Ceramide (c), sphingomyelin (e) and hexosylceramide (g) species and ratios of C16:0/C24:1 ceramides (d), sphingomyelins (f) and hexosylceramides (h) in islets of 8 male control and 8 ob/ob.B6 mice at week 12. i, j, Body weight (i) and blood glucose levels (j) of 12 female control and 11 Akita mice at week 7. k-p, Sphingolipid measurements as described in (c-h) in islets of 8 female control and 6 Akita mice at week 7. q, Percent increase in the C16:0/C24:1 ratio of specific sphingolipids in islets of male db/db.BKS, female Akita and male ob/ob mice to their respective controls (calculations based on lipidomics analyses shown in Fig. 1 and Extended Data Fig. 1). r, s, Body weight (r) and blood glucose levels (s) of 9 male control and 10 db/db.BKS mice at week 12 which were used for analyses shown in Fig. 1j-o. t, Volcano plot showing log2 fold change of baseline lipid measurements (shown in Fig. 1j-o) plotted against the –log10 p-value of a two-sided equal variance t-test. Cutoffs for significance as in Fig. 1d, e (n = 3 control vs. 3 db/db.BKS islet replicates). Only lipids detected in all samples were used for calculation. Statistics were performed using a two-sided Student’s t-test in (a, b, d, f, h-j, l, n, p, r, s) and multiple two-sided t-tests with Holm-Sidak correction in (c, e, g, k, m, o). Data points in (a-p, r, s) represent individual mice. Bar graphs in (a-p, r, s) represent means + s.e.m. Bar graphs in (q) represent values based on mean calculations of the respective groups presented in Fig. 1 and in this Figure. Source numerical data are available in source data.
