Extended Data Fig. 7: Phase separation diagram of EB1 from different species in vitro.
From: Phase separation of EB1 guides microtubule plus-end dynamics
Summary of in vitro phase separation behavior of wild-type and lysine/arginine mutated EB1 in response to changes in salt concentration. Proteins of both wild-type and mutated EB1 from different species were purified and assessed for in vitro phase separation propensity as described in Methods. The saturation concentration was measured by turbidity assay at indicated salt and protein concentrations. The red circles indicate phase separation (above saturation concentration), and the blue circles indicate no phase separation (below saturation concentration). Data are representative from three independent replicates.
