Extended Data Fig. 10: Dynamic phase separation of EB1 guides chromosome movements.
From: Phase separation of EB1 guides microtubule plus-end dynamics
(a) Characterization of expression levels of GFP-tagged EB1 wild type, KR6Q mutant and EB1-hnRNPA1 chimeras in endogenous EB1-depleted HeLa cells were analyzed by Western blot with an anti-EB1 antibody. Doxycycline (Dox) was added to induce EB1 knockout. (b) Representative mitotic phenotypes in endogenous EB1-depleted HeLa cells expressing EB1-hnRNPA1 chimeras shown by time-lapse microscopy. Chromosomes were visualized with mCherry-H2B. Arrows, spindle poles; arrowheads, lagging chromosomes. Scale bar, 10 μm. (c) Quantification of mitotic phenotypes of live HeLa cells in (b). For each group, n = 30 cells pooled from three independent experiments. Data represent mean ± s.e.m. Statistical significance was assessed by two-way ANOVA. ****p < 0.0001; ***p = 0.001; **p = 0.0041; *p = 0.0107; ns (not significant) indicates p > 0.05.
