Extended Data Fig. 8: Characterization of surface markers and NOTCH1 signaling in PD-1+ LSCs.
a,b, Quantification of T-ALL cells in peripheral blood (a) and survival curve (b) of recipients engrafted with indicated T-ALL cells (n = 5 mice). c, The expression, and quantification of indicated LSC markers (n = 4 mice). d, The survival curve of mice received the indicated numbers of T-ALL cells (n = 5 mice). e,f, Summary of limiting dilution experiments (f) and the competitive repopulating units (f). g, PD-1 expressioin in Lin–CD3+c-KitMid T-ALL cells in Pten null T-ALL. h, FACS analysis showing Lin, CD3, and c-Kit expression in PD-1+ and PD-1– Pten null T-ALL cells. i, The survival curve of T-ALL mice received the indicated T-ALL cells (n = 5 mice). j,k, Summary of limiting dilution experiments (j), and the competitive repopulating units (k). l, The expression, and quantification of indicated LSC markers in human T-ALL cells from recipients (n = 5 individual patients). m,n, Quantification of MYC-regulated gene expression in T-ALL cells (n = 3 mice). o, Quantification of indicated proteins (n = 3 mice) (Related to Fig. 5l). p,q, Western blots for T-ALL cells from Pten null T-ALL (p) and human T-ALL (q). Three individual mice or human samples were presented. r,s, Quantification of MYC regulated genes in human T-ALL cells (n = 3 individual patients). t, The NOTCH1 mutation rate in human T-ALL cells (n = 7 individual patients). u,v, Western blots (u) and correlation analysis (v) for indicated proteins in human T-ALL cells. 12 individual patient samples were presented. w, Relative PD-1 mRNA levels in indicated cells with NOTCH1 inhibitor (LY411575) treatment for 72 h (n = 3 mice). β-actin was used as a loading control (p,q,u). The P values were determined by the unpaired two-tailed Student’s t-test (a,c,l,m,n,o,r,s,t,w), Kaplan–Meier survival analysis (b,i), the ELDA platform (f,k), and pearson correlation analysis (v). All data are shown as mean ± s.d.
