Extended Data Fig. 6: Targeting Menin drives expression of bivalent genes independently of IFN and NF-κB signalling.
From: Targeting Menin disrupts the KMT2A/B and polycomb balance to paradoxically activate bivalent genes
a,b, Immunoblot in K-562 EED-KO cells depleted of MEN1 and PSIP1 (a) or PCGF1 (b) and then transduced with the indicated sgRNA. c, Immunoblot in K-562 Cas9 and EED-KO cells transduced with the indicated sgRNA and treated ± 10 ng ml−1 IFN-γ for 48 h. d–h, K-562 EED-KO cells depleted of MEN1, PSIP1 or PCGF1 and transduced with the indicated sgRNA, analysed by flow cytometry (d,f), and immunoblot (e,g,h). i, Immunoblot of K-562 Cas9 and EED-KO cells transduced with the indicated sgRNA and treated ± 20 ng ml−1 TNF-α for 48 h. j, Cell surface MHC-I expression in K-562 EED-KO cells transduced with control or PCGF1 sgRNA and treated ± 25 ng ml−1 IFN-γ for 24 h. d,f,j, Representative plots from three experiments (Supplementary Fig. 3).
