Extended Data Fig. 4: YAP-M and YAP-C fusion proteins form liquid-like nuclear condensates.
From: Nuclear condensates of YAP fusion proteins alter transcription to drive ependymoma tumourigenesis
a, Sections of cortical tumor tissues from mice transduced with YAP-M or YAP-C immunostained for YAP. Scale bar, 10 μm. b, 293 T cells transfected with empty vector or vectors expressing YAP, YAP-S112A, YAP-NLS, YAP-S112A-NLS, YAP-M, and YAP-C fusions immunostained for YAP (red). DAPI (blue) was used to stain nuclei. Scale bar, 5 μm. c, C2C12 cells transfected with YAP-M construct. Scale bar, 20 μm (left) and 5 μm (Right). d, Quantification of nuclear fluorescence intensity of the cells transfected with YAP-derivatives. Data shown are means; n = 30 cells for each group, pooled from 2 independent experiments. one-tailed unpaired t-test. e,f, YAP western blot analysis (e) and quantifications (f) showing the nuclear levels of YAP-NLS, YAP-M or YAP-C. Histone H1, loading control. n = 2 independent experiments with similar results. g, Live-cell imaging of C2C12 cells expressing YAP-C-GFP. The arrows indicate representative YAP-C nuclear puncta that fused over time. This assay was performed twice (two independent transfections) with similar results. Scale bar, 5 μm. h, Time-lapse images of YAP-M-GFP or YAP-C-GFP after 5% 1,6-hexanediol treatment.
