Extended Data Fig. 9: YAP-M fusion induces long-range super-enhancer interactions to promote oncogenic signaling.
From: Nuclear condensates of YAP fusion proteins alter transcription to drive ependymoma tumourigenesis
a, Circos map of combined omic profiles of YAP-M tumors. The innermost layer represents oncogenic genes near super-enhancers (SEs). The second layer includes SEs (n = 627). The third layer includes all enhancers (n = 16,381). The fourth layer represents the top 10,000 binding loci of YAP. The fifth layer includes differentially expressed genes in tumors from mice transfected with the YAP-M expression construct relative to NSCs that express YAP. The sixth layer represents the mouse genome (mm10). b, Distribution of H3K27ac binding genomic regions in the indicated cells. c, YAP-M fusion induces long-range enhancer/promoter interactions at the gene loci associated oncogenic signaling. Genomic tracks depicting long-range enhancer/promoter interactions at the indicated loci of oncogenesis-promoting genes in the control and YAP-M transduced NSCs. Pink arcs, DNA contacts; histograms, YAP1 and H3K27ac ChIP-seq peaks. d,e, Co-staining of HA-tagged YAP-M or YAP-MΔIDR (green) and H3K27ac (red) and immunoblot analysis in C2C12 cells transfected with YAP-M or YAP-MΔIDR constructs. Scale bars, 10 μm.
